PCR$505072$ - translation to german
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  3. PCR$505072$

PCR$505072$ - translation to german

TYPE OF PCR
Touchdown PCR; Touchdown pcr; Touch-down pcr
  • Scheme of touchdown PCR. The first annealing between primer and DNA occurs at the initial temperature, the second after the temperature has been lowered by <math>\Delta T</math>. Therefore, the product of the second annealing is disadvantaged by <math>2^{-1}</math>.

PCR      
Polymerase Kettenreaktion, labortechnik zur Vervielfachung von DNS Segmente (Molekularbiologie)
polymerase chain reaction         
  • "Baby Blue", a 1986 prototype machine for doing PCR
  • Exponential amplification
  • A [[thermal cycler]] for PCR
  • Mother}}<br />The child has inherited some, but not all, of the fingerprints of each of its parents, giving it a new, unique fingerprint.
  • 1024x438px
  • Diagrammatic representation of an example primer pair. The use of primers in an in vitro assay to allow DNA synthesis was a major innovation that allowed the development of PCR.
  • An older, three-temperature [[thermal cycler]] for PCR
  • Tucker PCR
  • DNA samples are often taken at crime scenes and analyzed by PCR.
IN VITRO METHOD FOR PRODUCING LARGE AMOUNTS OF SPECIFIC DNA OR RNA FRAGMENTS FROM SMALL AMOUNTS OF SHORT OLIGONUCLEOTIDE PRIMERS
Polymerase Chain Reaction; PCR oil; PCR amplification; Polymerase chain; Hot-start; PCR reaction; Molecular Xeroxing; Polymerase chain reacton; Applications of PCR; Mechanism of PCR; Examples of PCR; Nucleic Acid Amplification; Pcr; Applications of pcr; P.C.R.; Nucleic acid amplification; Pcr test; Single Specific Primer-PCR; SSP-PCR; PCR test; Polymerase chain reaction test; PCR testing
Polymerase Kettenreaktion, Methode zur Vervielfältigung von DNA durch wiederholte Teilung der DNA Stränge und ihre Vervielfältigung mit DNA polymerase Enzyme (Molekulare Biologie)

Definition

polymerase chain reaction
¦ noun Biochemistry a method of making multiple copies of a DNA sequence, involving repeated reactions with a polymerase.
Show more definitions

Wikipedia

Touchdown polymerase chain reaction

The touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers avoid amplifying nonspecific sequences. The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing. The melting point of the primer sets the upper limit on annealing temperature. At temperatures just above this point, only very specific base pairing between the primer and the template will occur. At lower temperatures, the primers bind less specifically. Nonspecific primer binding obscures polymerase chain reaction results, as the nonspecific sequences to which primers anneal in early steps of amplification will "swamp out" any specific sequences because of the exponential nature of polymerase amplification.

https://en.wikipedia.org/wiki/Touchdown polymerase chain reaction
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